Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes.

نویسندگان

  • T C McGuire
  • K I O'Rourke
  • T V Baszler
  • S R Leib
  • A L Brassfield
  • W C Davis
چکیده

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.

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عنوان ژورنال:
  • The Journal of general virology

دوره 75 ( Pt 4)  شماره 

صفحات  -

تاریخ انتشار 1994